needle takes two files as input. First file contains sequence to be aligned to the sequence or sequences in the second file. The first file can contain only one sequence and the second file can contain multiple sequences and needle will align the sequence from the first file to every sequence in the second file. Now coming to your question:

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Fri frakt ALIGNMENT GLOBAL. est2genome · needle · needleall · stretcher. ALIGNMENT LOCAL.

needle is a command line program from EMBOSS (“the European Molecular Biology Open Software Suite”). It is part of the "Alignment:Global" command group(s).

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Sekvenserna inriktades med användning av EMBOSS-Needle (//www.ebi.ac.uk/Tools/ psa / emboss_needle /). Sekvensidentiteten för dessa två proteiner är 61, EMBOSS Needle reads two input sequences and writes their optimal global sequence alignment to file. It uses the Needleman-Wunsch alignment algorithm to find the optimum alignment (including gaps) of two sequences along their entire length. needle reads two input sequences and writes their optimal global sequence alignment to file. It uses the Needleman-Wunsch alignment algorithm to find the optimum alignment (including gaps) of two sequences along their entire length. Needle finds an alignment with the maximum possible score where the score of an alignment is equal to the sum of the matches taken from the scoring matrix.
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ALIGNMENT.

The API reads the two input This program uses the Needleman-Wunsch global alignment algorithm to find the optimum alignment (including gaps) of two sequences when considering their Web Service EMBOSS needle (REST) REST · Gb · Protein Pairwise Alignment · Nucleotide Pairwise Alignment. % needle Needleman-Wunsch global alignment. Input sequence: embl:L07770 Second sequence(s): embl:U23808 Gap opening penalty [10.0]: Gap extension EMBOSS is the European Molecular Biology Open Software Suite. For the GEM-PRO pipeline, we mainly need the needle pairwise alignment tool ( although Jun 28, 2017 Needle program - This program uses the Needleman-Wunsch global alignment algorithm to find the optimum alignment (including gaps) of two EMBOSS is a free open source software analysis package developed for the needs of the molecular biology and bioinformatics user community.
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needle is a command line program from EMBOSS (“the European Molecular Biology Open Software Suite”). It is part of the "Alignment:Global" command group(s). OPTIONS¶ Input section¶-asequence sequence

Now coming to your question: needleall reads in two nucleotide or protein sequences inputs.